Karol <j...@W...gazeta.pl> napisał(a):



> http://linkinghub.elsevier.com/retrieve/pii/S0145212
60600049X

widze, ze tekst abstarktu pod podanym linkiem nie wyswietlil sie, wiec go
wklejam (niestety w j.ang.):


Multiple myeloma is malignant disease that is characterized in most patients,
by the presence in the serum of monoclonal gamma globulins, which in agarose
gel after electrophoresis appear as protein band of restricted mobility,
&#8220;M&#8221;
component.

The aim of this study was to determine are the antibodies contained in
M-component directed to some antigen chronically present in the organism, to
some of food antigens.

Seventeen patients with secretory plasmacytoma were included in the study:
eight of them had IgG(kappa), three had IgG(lambda), and one had biclonal
IgG(kappa) and IgA(kappa), while two had IgA(kappa), the other two IgA(lambda)
and one IgM(lambda) as paraproteins. M-proteins were detected analyzing
patients&#8217; sera by agarose gel electrophoresis in 0.09M barbital buffer. The
each M-protein was confirmed by immunotyping (immunofixation) with
corresponding antihuman antibodies directed to heavy or light chains of
immunoglobulins. After the patients serum separation on agarose gel by
electrophoresis, fresh 0.4% solution of crude gliadin (Sigma) in 1% SDS was
put over the slides for immunoprecipitation.

Preliminary results showed the interaction of gliadin with patient's serum
proteins present in the protein fraction of the same mobility as it was the
mobility of the M-component, in 6 from 17 investigated sera.

These results are the first reporting that in sera of some patients with
multiple myeloma antibodies from M-component could be directed to some of
gliadin antigens.

As the serum antigliadin immunoreactivity is present in patients with gluten
intolerance, celiac disease, it could be of importance to elucidate is the
multiple myeloma more severe form of gluten intolerance than celiac disease.


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